Figure 5. The effect of tHGA on the release of TNF-α by DENV3-induced RBL-2H3 cells.

RBL-2H3 cells were pretreated with tHGA (1.25, 5, and 20 μM) for 20 min and then infected with DENV3 at 0.2, 0.4, 0.8, and 1.6 × 10⁻² FFU/cell for 24 h. The level of TNF-α was determined using an ELISA kit by following the manufacturer’s instructions. Results are expressed as the mean ± S.E.M values of three independent experiments. ***P<0.005 as compared with the DENV3-induced RBL-2H3 group (black bars).