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. 2019 May 22;42(1):301–312. doi: 10.3892/or.2019.7166

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Copyright: © Cheng et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — (A-C) Validation of CK18-regulated genes (DEGs) by quantitative PCR and (C) western blotting in (A and C) HeLa cells, and in (B) HepG2 cells. (B) The effectiveness of three different CK18-specific shRNAs (left) and the effect of multiple CK18-specific shRNAs on the expression of four CK18-regulated genes (right) are presented. (C) Validation of the effect of CK18-specific shRNA1 on the protein level (left) and mRNA level (right) of CASP8. Experimental data are presented as the mean ± standard deviation of at least three experiments. *P<0.05 and **P<0.01. CK18, cytokeratin 18; DEGs, differentially expressed genes.