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. 2019 Jun 4;39(6):BSR20182366. doi: 10.1042/BSR20182366

Figure 3. The SFRP1 expression is inhibited by miR-27a in HOS and U2OS cells.

Figure 3

(A) miR-27a expression in HOS cells among seven groups detected by RT-qPCR. (B) miR-27a expression in U2OS cells among seven groups measured by RT-qPCR. (C) mRNA expression of SFRP1 in HOS cells among seven groups evaluated by RT-qPCR. (D) mRNA expression of SFRP1 in U2OS cells among seven groups determined by RT-qPCR. (E) Protein bands of SFRP1 and GAPDH in HOS cells among seven groups examined by Western blot analysis. (F) Protein bands of SFRP1 and GAPDH in U2OS cells among seven groups measured by Western blot analysis. (G) Protein expression of SFRP1 in HOS cells among seven groups examined by Western blot analysis. (H) Protein expression of SFRP1 in in U2OS cells among seven groups examined by Western blot analysis. The measurement data were expressed as mean ± S.D. The comparison among multiple groups was analyzed by one-way ANOVA; the experiment was repeated three times. *P<0.05 compared with the control inhibitor group; #P<0.05 compared with the control mimic group; &P<0.05 compared with the miR-27a inhibitor + si-NC group. Abbreviation: GAPDH, glyceraldehyde-3-phosphate dehydrogenase.