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. 2019 Jun 4;39(6):BSR20182366. doi: 10.1042/BSR20182366

Figure 6. miR-27a silencing inactivates the Wnt/β-catenin signaling pathway by up-regulating SFRP1.

Figure 6

(A) Activities of the Wnt/β-catenin signaling pathway in HOS and U2OS cells detected by immunofluorescence staining (×400). (B) Ratio of TOP-Flash Luc/Rel in both HOS and U2OS cells among seven groups detected by TOP-FLASH reporter assay. (C) The protein bands of VEGF, c-Myc, cyclin D1 and GAPDH in HOS and U2OS cells among seven groups detected by Western blot analysis. The measurement data were expressed as mean ± S.D. The comparison among multiple groups was analyzed by one-way ANOVA; the experiment was repeated three times; *P<0.05 compared with the control inhibitor group; #P<0.05 compared with the control mimic group; &P<0.05 compared with the miR-27a inhibitor + si-NC group. Abbreviation: GAPDH, glyceraldehyde-3-phosphate dehydrogenase.