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. 2019 Jun 3;2(3):e201800261. doi: 10.26508/lsa.201800261

Figure 4. Increased RNA Pol III occupancy at tRNA genes is observed in hmt1Δ and Rpc31R5,9A cells under stress.

Figure 4.

(A, B) The in vivo occupancy across the four tRNA genes for Rpc82 (A) and Rpc160 (B) was determined by ChIP. qPCR results for products of ChIP performed on WT, hmt1Δ, orRpc31R5,9A cells before and after treatment of CPZ are displayed as bar graphs. Percentage of input is calculated by ΔCT. The error bars representing SEM of three biological samples (n = 3). P-value as calculated by t test: *<0.05; **<0.01, and ***<0.001. (C) Fold decrease in Rpc82 and Rpc160 occupancy for four candidate tRNA genes in WT, hmt1Δ, or Rpc31R5,9A cells after treatment with CPZ. qPCR was performed for products of ChIP on WT, hmt1Δ, or Rpc31R5,9A cells before and after treatment with CPZ. Percentage of input is calculated as ΔCT. ANOVA on these values yielded significant variation among the three strains (P-value = 0.0015). Post hoc Tukey test revealed significant differences between WT and hmt1Δ cells (adjusted P-value is 5.4 × 10−4), and between WT and Rpc31R5,9A cells (adjusted P-value is 9.8 × 10−4). n = 3 per tRNA gene.