Figure S1. RNA hybridization data from WT, hmt1Δ, and Rpc31^R5,9A cells before and after CPZ treatment.

Total RNAs were extracted from WT, hmt1Δ, and Rpc31^R5,9A cells before and after CPZ treatment. The media used to grow the cell in each panel are stated. 10 μg of RNA from each biological sample is loaded and resolve by an 8% urea-TBE gel. Resolved RNAs were transferred onto nylon membrane and subjected to RNA hybridization using radiolabeled oligonucleotide probes corresponding to pre-tRNAs of tL(CAA), tL(UAG), tP(UGG), and tY(GUA). (A, B) As a control for loading, the blots were also probed for U3, U4, and U5. Sample hybridization data from WT versus hmt1Δ (A) or versus Rpc31^R5,9A (B) before and after CPZ treatment.