Skip to main content
. 2019 Jun 3;2(3):e201800288. doi: 10.26508/lsa.201800288

Figure 4. The F-BAR and first linker region are required for membrane binding and bending.

Figure 4.

(A) Schematic of deletion constructs of CIP4S and FBP17L. (B) Images of living cortical neurons cotransfected with mRuby-Lifeact and EGFP-labeled protein or deletion mutant at 12 h postplating. (C–F) Quantification of stage 1 neurons comparing the effects of the deletion constructs on peripheral intensity (C), filopodia number (D), cell complexity (E), and tubule number (F) at 12 h postplating. CIP4S-EGFP (n = 24 cells), C-CCC-EGFP (n = 35 cells), C---- EGFP (n = 21 cells), CCS--- EGFP (n = 22 cells), FBP17L-EGFP (n = 23 cells), F-FFF EGFP (n = 33 cells), F---- EGFP (n = 28 cells), and FFL--- EGFP (n = 29 cells). One-way ANOVA with Kruskal–Wallis post-test multiple comparisons. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001; ns, not significant. Scale bars represent 5 µm in whole-cell images and 1 µm in insets.