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. 2019 Jun 3;2(3):e201900315. doi: 10.26508/lsa.201900315

Figure S3. Multimodal and very long telomeres in strains CC503+ and cw15.J14+ correspond to terminal DNA fragments. Related to Fig 3.

Figure S3.

(A) TRF Southern blot of biological replicates of the indicated strains shown in Fig 3 (left), with intensity profile analysis (right) showing the multimodal distribution of CC503+ and CC1010+. (B) gDNAs of strains CC503+ and cw15.J14+ were subjected to Bal31 exonuclease digestion for 1 to 10 min before digestion by the restriction enzyme cocktail and Southern blotting and hybridizing using the CHSB (oT0958) probe, showing that the detected signal is indeed Bal31-sensitive and, hence, represents terminal fragments. Asterisk: partially degraded DNA sample; refer to lane “NP” for the nontreated control.