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. 2019 May 29;10:1210. doi: 10.3389/fmicb.2019.01210

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Copyright © 2019 Ospino, Kojima and Fukui.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Positions of PCR primers for the arxA gene and expected size of the PCR product. (B) Gel images showing the results of PCR with newly designed primers. Samples in lanes are: M, 10 kb DNA ladder as a size marker; (1–2) Jozankei microbial mat; (3) Sulfuritortus calidifontis J1A; (4) Nitrincola lacisaponensis 4CA; (5) Sulfuricella denitrificans skB26; (6) no template control; (7) Mizugaki water; (8) Alkalilimnicola ehrlichii MLHE-1; (9) Sulfuritalea hydrogenivorans sk43H; (10) no template control. In lanes 1 and 2, bands of expected sizes are marked with triangles.