Fig. 5.

Activation of grafted cells by locomotor training. a Representative images of cells expressing CTIP2, SATB2, PAX6, TBR1, and CUX1 in the GFP⁺ graft in the TMT (+) group. Scale bar, 50 µm. Nuclear counter staining, 4,6-dimamidino-2-phenylindole (DAPI). Quantitative analysis of cells expressing CTIP2, SATB2, PAX6, TBR1, and CUX1 in the graft showed there were no differences in the percentages of CTIP2⁺ cells, SATB2⁺ cells, PAX6⁺ cells, TBR1⁺ cells, and CUX1⁺ cells per DAPI⁺ cells between the TMT (−) group and TMT (+) group. Mann–Whitney tests were performed; n = 4 in the TMT (−) group and n = 8 in the TMT (+) group. Data are presented as means ± SEM. b Representative images of double-labeled stained cells of C-FOS⁺ and GFP⁺ cells in the graft. Scale bars, 50 µm. Nuclear counter staining, DAPI. Quantitative analyses of the cells expressing C-FOS in the graft showed the percentage of C-FOS⁺ cells was significantly higher in the TMT (+) group (2.08 ± 0.57%) than in the TMT (−) group (0.36 ± 0.12%). Mann–Whitney tests were performed; n = 4 in the TMT (−) group and n = 8 in the TMT (+) group. Data are presented as means ± SEM