Table 2.
Differential gene expression induced by exposure to osteogenic differentiation media for 10 days.
| Up-regulated genes | Down-regulated genes | ||||
|---|---|---|---|---|---|
| COB-ODM/COB-GM | COB-ODM/COB-GM | ||||
| Gene | Fold Reg. | p-value | Gene | Fold Reg. | p-value |
| Ahsg | 3.6 | 0.0045 | Bmp4 | −973.8 | <0.0001 |
| Cdh11 | 3.1 | <0.0001 | Col10a1 | −7.8 | <0.0001 |
| Col4a1 | 2.9 | 0.0018 | Col1a1 | −4.6 | 0.0033 |
| Fgfr2 | 5.0 | 0.0013 | Col1a2 | −2.5 | 0.0113 |
| Flt1 | 5.2 | 0.0004 | Col2a1 | −5.7 | 0.0142 |
| Gdf10 | 10.5 | 0.0008 | Col5a1 | −2.5 | 0.0017 |
| Gli1 | 3.5 | 0.0181 | Fn1 | −2.5 | 0.0043 |
| Mmp10 | 4.3 | 0.0001 | Igf1 | −3.2 | 0.0021 |
| Smad5 | 2.7 | 0.0426 | Itgam | −10.3 | 0.0013 |
| Spp1 | 4.3 | <0.0001 | Phex | −4.9 | 0.0003 |
| Tgfbr3 | 8.5 | 0.0285 | Tgfbr1 | −2.4 | 0.0027 |
| Vcam1 | 2.7 | <0.0001 | Tnf | −15.0 | 0.0023 |
| Gapdh | −2.5 | 0.0067 | |||
| Clone4-ODM/Clone4-GM | Clone4-ODM/Clone4-GM | ||||
| Bglap | 29.4 | 0.0476 | Col3a1 | −2.1 | 0.0425 |
| Bmp4 | 2.6 | 0.0100 | Fgf2 | −2.6 | 0.0168 |
| Col2a1 | 2929.6 | 0.0366 | Nog | −3.3 | 0.0130 |
| Igf1r | 534.9 | 0.0061 | Serpinh1 | −2.9 | 0.0275 |
| Itga2 | 2.3 | 0.0366 | Tgfbr2 | −2.2 | 0.0130 |
| Phex | 6.4 | 0.0091 | |||
| Sp7 | 2.3 | 0.0181 | |||
| Spp1 | 6.1 | 0.0028 | |||
| Tgfb1 | 2.9 | 0.0199 | |||
| Clone14-ODM/Clone14-GM | Clone14-ODM/Clone14-GM | ||||
| — | — | — | Alpl | −6.2 | 0.0185 |
| Clone24-ODM/Clone24-GM | Clone24-ODM/Clone24-GM | ||||
| Bglap | 14.4 | 0.0016 | Fgf2 | −2.2 | 0.0281 |
| Col2a1 | 54.1 | <0.0001 | Igf1 | −4.1 | 0.0031 |
| Csf1 | 2.6 | 0.0003 | Tgfbr2 | −2.8 | 0.0017 |
| Phex | 11.8 | 0.0018 | |||
| Spp1 | 5.0 | 0.0297 | |||
| Vdr | 2.7 | 0.0061 | |||
| Vegfa | 2.8 | 0.0318 | |||
Data shown if >2-fold up- or down-regulated and p ≤ 0.05 by Student’s t-test. COB = primary calvarial osteoblast, ODM = osteogenic differentiation media, GM = growth media.