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. 2019 Jun 5;51(6):65. doi: 10.1038/s12276-019-0264-9

Fig. 4. Mutant huntingtin aggregation in the brain tissue of six-month-old zQ175 mice is reduced by metformin treatment.

Fig. 4

a Immunohistochemical (IHC) staining illustrates the expression levels of mutant huntingtin (mHTT) in the cortex and striatum of zQ175 mice and their corresponding controls (wild type (WT)). Representative images of each condition are shown (bars: 50 µm). Boxed areas are enlarged, with a higher magnification, on the right side of each image. b The bottom panel shows a semiquantitative analysis of IHC staining using the ImageJ software. Samples from four independent mice from each group were analyzed, which generated 10 cortex and 8 striatum pictures from untreated zQ175 (Huntington disease (HD)) mice and 10 cortex and 7 striatum pictures from HD-treated (HD metformin) mice. The analyzed area in each picture was 90,488 μm2. HD mice treated with metformin showed fewer mHtt aggregates (p = 0.00243), and the reduction in the aggregates was more pronounced in the striatum of these mice (p < 0.001). Values are the mean, and bars indicate the confidence interval (95% CI)