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. 2019 Jun 5;9:8306. doi: 10.1038/s41598-019-44220-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phylogenetic trees of HA H5, H7, H9, and NA N2 and N1 of sequences established from clinical samples (green) and plaque-purified viruses (red) from Tangail district. Sequences of samples from Mymensingh district are shown in blue. Maximum likelihood trees were established using the IQ-Tree software. Amino acid alignments of the HA1 and the full length NA proteins were used for calculation based on the specific FLU substitution model recommended by Dang et al.⁴⁴. Robustness of branching orders was tested by an ultrafast bootstrap approximation algorithm³⁸. Trees are drawn to scale and clading information is shown to the right of each tree.