Fig. 5. Overexpression of miR-384 impaired Hoxa13-induced attenuation of chronic cerebral ischemia (CCI)-induced neuronal apoptosis by targeting 3´-UTR of Hoxa13.

a The predicted miR-384-binding site in Hoxa13 and the designed mutant sequences were indicated. Dual-luciferase report assay of HT22 cells cotransfected with Hoxa13-Wt and miR-384-NC or Hoxa13-Wt and miR-384, and Hoxa13-Mut and miR-384-NC or Hoxa13-Mut and miR-384 (n = 4, each group). Data are presented as mean ± SD, *P < 0.05 vs. Hoxa13-Wt+miR-384-NC group. b Flow cytometry analysis of CCI-induced HT22 cells cotransfected with miR-384 and Hoxa13/Hoxa13- (non-3´UTR) (n = 4, each group). Data are presented as mean ± SD, **P < 0.01 vs. miR-384+Hoxa13-NC group, ^#P < 0.05 vs. miR-384+Hoxa13 group. c FAM3A protein levels in CCI-induced HT22 cells cotransfected with miR-384 and Hoxa13/Hoxa13-(non-3´UTR) (n = 4, each group). Data are presented as mean ± SD, *P < 0.05 vs. miR-384+Hoxa13-NC group, ^#P < 0.05 vs. miR-384+Hoxa13 group