Fig. 1. Loss of TG2 does not affect weight gain during diet-induced obesity, but enhances the pro-inflammatory cytokine production by the gonadal white adipocytes and adipose tissue macrophages.

a Body weights of wild type and TG2 null mice fed on normal (ND), high-sucrose/low-fat (HSD) or high-sucrose/high-fat (HFD) diet at the end of the feeding period. b Gonadal fat weights of wild type and TG2 null mice fed on ND, HSD or HFD at the end of the feeding period. c Adiponectin, resistin, TNF-α, MCP-1, and leptin relative gene expression levels of gWAT adipocytes in wild type and TG2 null mice fed on ND, HSD or HFD determined by qRT-PCR at the end of the feeding period. GAPDH was used as reference gene. d TG2, IL-1β, IL-6, TNF-α, MCP-1, and resistin relative gene expression levels of gWAT macrophages in wild type and TG2 null mice fed on ND, HSD or HFD determined by qRT-PCR at the end of the feeding period. GAPDH was used as a reference gene. Results are expressed as mean ± SD (n = 8 mice per group). Statistical significance was evaluated by one-way ANOVA test (*p < 0.05)