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. 2019 Jun 5;10(6):439. doi: 10.1038/s41419-019-1677-z

Fig. 5. LXR agonist treatment reverts HFD-induced obesity with less hepatic steatosis in mice lacking TG2 in the bone marrow- derived cells.

Fig. 5

a BMI of BoyJ mice transplanted with the bone marrow of either TG2+/+ or TG2−/− mice at the end of the 16-week HFD feeding period combined or not with the LXR agonist GW3965 (20 mg/kg/day) treatment. b gWAT weights of the same mice. c Relative gene expressions of Bid, Bim, and adiponectin of gWAT adipocytes from the same mice determined by qRT-PCR using GAPDH as a reference gene. d Confocal images of gWAT collected from the same mice. Paraffin-embedded gWAT slides were stained with the non-specifically labeling anti-digoxin antibody, anti-F4/80 antibody and DAPI to visualize adipocytes, macrophages, and nuclei under confocal microscopy. Scale bar, 100 μm. CLS cells in fields from randomly selected sections of three different mice in each group were quantified. Results are expressed as mean ± SD (n = 3 mice per group). Statistical significance was evaluated by 2-tailed unpaired Student’s t-test (*p < 0.05). e Liver weights of the same mice. f Liver triacylglycerol contents from the same mice determined from saponified, neutralized liver extracts by glycerol enzymatic assay. g Paraffin-embedded liver tissue slides from the same mice stained with H&E to visualize tissue architecture. One representative series of three are shown. Scale bar, 250 μm. h MCP-1, IL-6, and resistin relative gene expression levels of gWAT macrophages from the same mice determined by qRT-PCR using GAPDH as a reference gene. i Serum insulin levels determined by Mouse Insulin ELISA kit. j Insulin resistance values of the same mice. Insulin resistance test was performed on week 15 (6 h fasting followed by intraperitoneal administration of 0.75 IU/bwkg insulin). Data are presented as mean ± SD (n = 8 mice per group). Statistical significance was evaluated by two-way ANOVA (*p < 0.05)