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. 2019 May 29;7:87. doi: 10.3389/fcell.2019.00087

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Copyright © 2019 Song, Laureano, Patel, Yip, Jadali and Kwan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Changes in transcript and protein levels in proliferating iMOP and iMOP-derived neurons. Relative changes in (A) cell cycle genes (Cdk2, Cdkn1a, and Cdkn1b) and (B) transcription factors (Sox2, Neurog1, and Neurod1) using quantitative PCR (qPCR) in proliferating iMOP (black) and iMOP-derived neurons cultured in the presence of 1 μM K03861 (white) (n = 3 independent experiments, ^∗∗p < 1 × 10^-2 and ^∗∗∗p < 1 × 10^-3). (C) Western blot of SOX2, NEUROD1, and ACTB at different time points during neuronal differentiation. (D) SOX2 and NEUROD1 protein levels normalized to ACTB at different time points (n = 3 independent experiments).