Table 1.

Primers used on conventional PCR assays.

Species	Primers (5′ → 3′)	AS (bp)
OMMV and/or TNV-D	Fw: GTGTTCAGTCATATACATACC	247
	Rv: GCCTATTGTGCTGTACCAC
OLV-1	Fw: TTTCACCCCACCAAATGGC	747
	Rv: CTCACCCATCGTTGTGTGG
OLYaV	Fw: CGAAGAGAGCGGCTGAAGGCTC	346
	Rv: GGGACGGTTACGGTCGAGAGG

Description of forward (Fw) and reverse (Rv) primers used for specific detection of Olive mild mosaic virus (OMMV) and/or Tobacco necrosis virus D (TNV-D) (Cardoso et al., 2004), Olive latent virus 1 (OLV-1) and Olive leaf yellowing-associated virus (OLYaV) in the conventional PCR assays (Zellama et al., 2018). AS, amplicon size.