Table 2.
Species | Accession ID | Primers (5′ → 3′) | AS (bp) | References |
---|---|---|---|---|
OMMV and/or TNV-D | AY616760 | Fw: GTGTTCAGTCATATACATACC | 247 | Cardoso et al. (2004) |
Rv: GCCTATTGTGCTGTACCAC | ||||
OLV-1 | KF804054 | Fw: GGGGTATGATGGTGCTATGG | 162 | This work |
Rv: ACTCCGCAATATCCGTTCTG | ||||
OLYaV | AJ844555 | Fw: GCTTATCTACTACGCCGATCTTGTC | 71 | This work |
Rv-AAGAGTGGATCCATCTAGATCGAAA | ||||
Description of forward (Fw) and reverse (Rv) primers used for specific detection of Olive mild mosaic virus (OMMV) and/or Tobacco necrosis virus D (TNV-D), Olive latent virus 1 (OLV-1) and Olive leaf yellowing-associated virus (OLYaV), in the qPCR assays. AS, amplicon size.