Table 3.

Detection limits of each of the three qPCR assays based on determination of copy numbers.

Virus	Cq value	Copy number
TNV-D/OMMV	33.27	0.8
OLV-1	33.36	0.8
OLYaV	32.07	8

The detection range was determined from standard curves generated from the dilution series of respective recombinant plasmid DNA. The mean Cq values of positive samples were represented with respective copy numbers. TNV-D, Tobacco necrosis virus D; OMMV, Olive mild mosaic virus; OLV-1, Olive latent virus 1; OLYaV, Olive leaf yellowing-associated virus.