Fig. 7.

Diagram showing the in vitro culture system for the bioartificial heart. a Mixed gas tank used for gas supply in organ culture. The main component is a mixture of oxygen and carbon dioxide. The amount of the mixed gas may be adjusted by the valve to ensure the percentage of oxygen in the mixed air, so that the medium supply ensures that the organ receives sufficient amount of oxygen during the cultivation process. b Three-hole glass bottle. The three holes are connected to the mixed gas, the inlet, and the outlet of the organ culture bottle through a hose. The medium containing saturated gas can be recycled through this bottle. c Disposable infusion tube heater. This heater can heat the culture medium pumped from the three-hole glass bottle at a constant temperature so that the medium entering the organ is maintained at an optimum temperature of 37 °C. d Thermostatic bath. This device has both heating and pumping functions, which can maintain the outer tube of the F device at 37 °C water circulation, thereby providing the optimum ambient temperature for the organ. e Titration pump. This device can continuously and quantitatively draw the culture medium from B into F, thereby ensuring that the organ is cultured in a nutrient-rich fresh medium. f Organ culture bottle. This device is divided into an outer tube and an inner tube, and these two are not connected. The outer tube is maintained at a constant temperature of 37 °C by connecting to a constant temperature bath. The organ is fixed by two hollow tubes of the cap and placed in the inner tube for cultivation. The entire system can be sealed to ensure a sterile environment