Table 2.
Food intake and serum hormonal and biochemical variables in rats fed different protein/carbohydrate ratios and exposed for 72 h to a cold environment
| Room temperature (23 °C) | Cold temperature (4 °C) | Protein (%) | Temperature (°C) | Interaction DP X T | |||||
|---|---|---|---|---|---|---|---|---|---|
| Protein content in the diet (%) | |||||||||
| 6 | 20 | 50 | 6 | 20 | 50 | ||||
| Δ Body weight (g) | 10.2 ± 1.5 | 17.3 ± 3.9 | 13.5 ± 6.7 | − 26 ± 9.6 | − 22.8 ± 4.8 | − 16.8 ± 6.6 | NS | < 0.0001 | NS |
| Food intake (g/d) | 32.9 ± 1.5 | 29.0 ± 3.2 | 30.6 ± 2.0 | 34.1 ± 1.9 | 34.2 ± 1.8 | 32.6 ± 1.0 | NS | NS | NS |
| Food intake (kcal) | 115.2 ± 5.3 | 101.5 ± 11.2 | 107.1 ± 7 | 119.4 ± 6.7 | 119.7 ± 6.3 | 114.1 ± 3.5 | NS | NS | NS |
| Protein intake (g/d) | 2.0 ± 0.1 | 5.8 ± 0.6 | 15.3 ± 1 | 2.1 ± 0.1 | 6.8 ± 0.4 | 16.3 ± 0.5 | < 0.0001 | NS | NS |
| Protein intake (kcal/d) | 8.0 ± 0.4 | 23.2 ± 2.6 | 61.2 ± 4 | 8.2 ± 0.5 | 27.3 ± 1.4 | 65.2 ± 2 | < 0.0001 | NS | NS |
| Glucose (mM) | 7.4 ± 0.12 | 8.1 ± 0.22 | 8.0 ± 0.14 | 8.6 ± 0.19 | 8.6 ± 0.3 | 8.5 ± 0.2 | NS | < 0.0001 | NS |
| Triacylglycerols (mM) | 2.2 ± 0.14 | 2.5 ± 0.31 | 1.81 ± 0.11 | 3.65 ± 0.23 | 3.95 ± 0.3 | 3.44 ± 0.92 | NS | < 0.0001 | NS |
| Fat body mass (%) | 12.3 ± 0.7 | 10.7 ± 1.1 | 11.8 ± 0.9 | 11.1 ± 2.5 | 10.2 ± 1.0 | 10.4 ± 2.2 | NS | NS | NS |
Values are the mean ± SEM, n = 5 rats per group. Protein-temperature interaction was evaluated by two-way ANOVA. Significant differences for % dietary protein, temperature, and the interaction between them (DP X T) are reported as significant (p < 0.05) or not significant (NS)