Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jun 4;50:43. doi: 10.1186/s13567-019-0660-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Analysis of secreted proteins. A SDS-PAGE profile of extracellular proteins. Lane 1: wild-type strain Yb2; lane 2: mutant strain Yb2∆gldM; lane 3: complementation strain cYb2∆gldM. Strains were incubated with shaking until cultures reached an OD₆₀₀ of 0.8. The same volume of concentrated cell-free supernatant from each strain was separated by SDS-PAGE, and proteins were visualized with Coomassie Brilliant Blue staining. B Determination of proteolytic activity. The error bars represent the standard deviation calculated from three independent experiments performed in triplicate (****p < 0.0001; ns, p > 0.05)