Table 3.
qPCR verification of differentially expressed genes in the mutant strain Yb2Δ gldM and complementation strain cYb2Δ gldM
| Gene locusa | Description of genes | 2−ΔΔCt | |
|---|---|---|---|
| Yb2ΔgldM | cYb2ΔgldM | ||
| AS87_RS00230 b | PorT protein | 3.5925 | 1.472 |
| AS87_RS02915 | Hypothetical protein | 2.445 | 1.3755 |
| AS87_RS09140 | Hypothetical protein | 2.0875 | 1.2058 |
| AS87_RS09480 | Twitching motility protein PilT | 2.06 | 1.2397 |
| AS87_RS02755 | Hypothetical protein | 2.0533 | 0.9432 |
| AS87_RS08665 | Tat pathway signal sequence domain-containing protein | 2.03 | 1.3947 |
| AS87_RS09585 | Hypothetical protein | 1.6533 | 1.1028 |
| AS87_RS08925 | SusC/RagA family TonB-liked outer membrane protein | 1.4225 | 1.6531 |
| AS87_RS08050 | Carbohydrate-bonding protein | 1.3875 | 0.8593 |
| AS87_RS04485 | Cbb3-type cytochrome oxidase component FixQ | 1.35 | 1.021 |
| AS87_RS07185 | Hypothetical protein | 1.28 | 1.0352 |
| AS87_RS09360 | Hypothetical protein | 1.2725 | 1.1408 |
| AS87_RS05780 | Hypothetical protein | 1.12 | 0.8526 |
| AS87_RS05060 | Hypothetical protein | 1.0733 | 0.7474 |
| AS87_RS10475 | Hypothetical protein | 1.0233 | 0.8745 |
| AS87_RS08045 | RagA family TonB-liked outer membrane protein | 0.985 | 1.2256 |
| AS87_RS00555 | RNA polymerase sigma24 factor | 0.98 | 1.0867 |
| AS87_RS08885 | Hypothetical protein | 0.947 | 1.591 |
| AS87_RS09380 | Hypothetical protein | 0.945 | 1.0497 |
| AS87_RS01535 | Hypothetical protein | 0.93 | 1.2226 |
| AS87_RS05020 | Hypothetical protein | 0.9175 | 0.895 |
| AS87_RS09995 | Hypothetical protein | 0.8825 | 1.4948 |
| AS87_RS08585 | ABC transporter | 0.8425 | 1.5157 |
| AS87_RS00880 | ABC transporter related protein | 0.6175 | 0.9659 |
| AS87_RS08470 b | Gliding motility protein GldN | 0.615 | 0.7813 |
| AS87_RS07850 | Hypothetical protein | 0.47 | 0.9075 |
| AS87_RS01360 | Hemolysin D | 0.34 | 0.5586 |
| AS87_RS01365 | Hypothetical protein | 0.33 | 0.8645 |
| AS87_RS01350 | Heavy metal efflux pump, CzcA family | 0.295 | 0.7846 |
| AS87_RS01355 | Transporter | 0.225 | 0.6925 |
aBased on the R. anatipestifer Yb2 genome (accession number: CP007204).
bThese genes encode T9SS components.