Figure 1.

Boron deficiency symptoms of NtXIP1;1 overexpressing N. tabacum lines. (a) NtXIP1;1 overexpressing (OE‐1 and OE‐2) and wild type (WT) N. tabacum plants. The OE‐1 and OE‐2 are branched and have several shoot apical meristems. Inset: Western blot showing the NtXIP1;1 protein level in young leaves of 5‐week‐old soil grown WT, OE‐1 and OE‐2 lines detected using an anti‐NtXIP1;1 antibody (upper picture), and coomassie blue staining of the corresponding microsomal membrane fraction (lower picture). (b) Typical leaf tissue malformations and shoot apical meristem phenotypes observed in 5–6 week old tobacco lines overexpressing NtXIP1;1. White arrows highlight malformed leaves and white arrowheads meristems that stopped their development