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. 2019 Jun 5;3(6):e00143. doi: 10.1002/pld3.143

Figure 7.

Figure 7

Phenotype and boron tissue concentration of Arabidopsis plants overexpressing NtXIP1;1. (a) Western blot of microsomal fractions of wild type Arabidopsis (Col‐0) and NtXIP1;1 overexpressing lines (OEA‐1 to ‐3) using the anti‐NtXIP1;1 antibody (upper panel). The monomeric and dimeric NtXIP1;1 forms are marked (black arrowheads). Coomassie staining of a SDS‐PAGE gel containing corresponding microsomal fractions, used as a loading control (lower panel). (b) Boron deficiency symptoms in young leaves of Arabidopsis lines overexpressing NtXIP1;1 (OEA‐1 to ‐3) in comparison to the Col‐0 wild type plant when grown on fully fertilized standard greenhouse soil substrate. Curled‐down and malformed B deficiency symptoms displaying young leaves are marked with white arrowheads. (c) Boron concentration of leaf samples of wild type Col‐0 Arabidopsis (black bar) and NtXIP1;1 overexpressing lines (OEA‐1 to ‐3) (gray bars) (of [b]) determined using ICP‐MS analysis. Asterisks indicate significant different B concentrations between the WT and the NtXIP1;1‐overexpressor lines (***p < 0.001; Student's t test). Values represent means ± SE (n = 4)