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. 2019 Jun 5;3(6):e00143. doi: 10.1002/pld3.143

Figure 8.

Figure 8

Partial growth complementation of the Arabidopsis Atnip5;1 knockout plants by tissue‐specific expression of NtXIP1;1. (a) PCR and RT‐PCR on leaf genomic DNA (gDNA) and root RNA, respectively, extracted of Arabidopsis wild type (Col‐0) or Atnip5;1 knockout plants and Atnip5;1 knockout lines transformed with the AtNIP5;1 pro :NtXIP1;1 construct, grown under B‐deficient in vitro growth conditions. PCR detected the genomic region of AtNIP5;1 or the T‐DNA insertion in the AtNIP5;1 genomic sequence. RT‐PCR detected NtXIP1;1 cDNA and the Elongation factor α (EFα) as a reference. (b) Partial rescue of the B deficiency phenotype of the Atnip5;1 knockout plants growing on standard soil substrate by the expression of NtXIP1;1 under the control of the AtNIP5;1 promoter in comparison to the wild type (Col‐0) and Atnip5;1 knockout plants. (c) Shoot dry weight of wild type (Col‐0) or Atnip5;1 knockout plants and independent Atnip5;1 knockout lines expressing the AtNIP5;1 pro :NtXIP1;1 construct. Bar charts represent means ± SD (n = 4). Significant differences in the shoot dry weight compared to Col‐0 are marked with an asterisk (***p < 0.001; Student's t test). (d) Leaf B concentration of wild type (Col‐0) or Atnip5;1 knockout plants and independent Atnip5;1 knockout lines expressing with the AtNIP5;1 pro :NtXIP1;1 construct. Bar charts represent means ± SD (n = 4). Significant differences in leaf B concentrations compared to Atnip5;1 knockout plants are assessed (**p < 0.01; **p < 0.001; Student's t test)