Figure 4.
SAS inhibits in vitro attachment of T and B lymphocytes from NOD mice to the high endothelial venular ('HEV') cells (SVEC-4). Cells were washed twice. Eighty percent confluent endothelial SVEC cells in 6 well plates were stimulated with TNFα to induce MAdCAM-1 expression. After 10 h, 3 × 106 splenocytes from 10 week old NOD mice were added in the presence or absence of various concentrations of SAS o.n. Culture plates were washed and the attached cells were removed and stained with FITC-conjugated anti CD3, and APC-conjugated anti B220 antibodies for the detection of attached T (A) or B (B) cells. The results represent one experiment, representative of three performed.