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. 2019 May 29;10:979. doi: 10.3389/fimmu.2019.00979

Figure 8.

Figure 8

Effect of the compounds on islet morphology and insulitis. AS101 or SAS at 1mg/kg/injection were administered i.p. every other day to female NOD mice, starting at 3 weeks of age. At 12 weeks (A) (beginning of insulitis) or at 20 weeks (B) (severe diabetes)., mice were starved O.N. and injected i.p. with 2gr/kg glucose. Blood glucose levels were monitored at different time points. **p < 0.01 vs. PBS; *p < 0.05 vs. PBS. N = 10/group. (C–F) At 20 weeks, pancreatic heads were fixed and stained with H&E. (C) Pre-diabetic mice islets at 5w. (D) PBS. (E) AS101 (1 mg/kg/injection). (F) SAS (1 mg/kg/injection). Results show representative data of five mice/group. X200. (G) Percent insulitis of 5 mice/group at 20 weeks in comparison to islets from 5 weeks old mice (pre-diabetic). 20 islets/mouse were scored. *p < 0.01 vs. PBS. (H) Percentage of infiltrating cells according to the severity of insulitis. Grade 0, No insulitis (no infiltration); Grade 1, Pre-insulitis (<25% of the islet area infiltrated); Grade 2, Mild insulitis (<50% of the islet area infiltrated); Grade 3, Severe insulitis (>50% of the islet area infiltrated); Grade 4, Massive insulitis (total loss of β cell mass).