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. 2019 May 28;12:4203–4211. doi: 10.2147/OTT.S196910

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© 2019 Yu et al.

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Prx-1/NF-κB/HIF-1α pathway is involved in the regulation of the expression of MCP-1: Lewis cells were cultured with radiation and hypoxia. Prx-1, HIF-1α, and p65 protein at different time points were analyzed by Western blot. (A) Lewis cells transfected with Prx-1, HIF-1α, or p65-specific siRNA or control siRNA (NSP), respectively, were treated with or without radiation and hypoxia, and then MCP-1 protein secretion was determined by ELISA assay. (B) Lewis cells transfected with Prx-1, HIF-1α, or p65 siRNA or control siRNA (NSP) were treated with or without radiation and hypoxia for 48 hrs, and then the expression of Prx-1, HIF-1α, and p65 was checked by Western blot. β -actin and Histone-H3 served as the loading control. The quantitative analysis of the proteins was performed, respectively. (C–E) Data were presented as the mean±SD of three independent experiments. The level of significance was indicated by *P<0.05, **P<0.01, ***P<0.001.