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. 2019 May 28;12:4203–4211. doi: 10.2147/OTT.S196910

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© 2019 Yu et al.

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β-elemene inhibits radiation and hypoxia-induced macrophages infiltration via inhibiting Prx-1/NF-κB/HIF-1α pathway: RAW264.7 cells cultured with conditioned medium were treated with or without β-elemene and then subjected to Matrigel invasion assay and photographed (magnification 20×, scale bar 100 µm) for the analysis of their invasion capacity. (A) Lewis cells cultured with irradiation (4 Gy) or hypoxia were treated with or without β-elemene. After 24 hrs, MCP-1 mRNA level was analyzed by qPCR. (B) MCP-1 protein secretion obtained from the Lewis cells supernatant was analyzed by ELISA. (C) Lewis cells were cultured with radiation and hypoxia and then were treated with or without β-elemene. The expression of Prx-1, HIF-1α, and p65 was analyzed by Western blot. β-actin and Histone-H3 served as the loading control. The quantitative analysis of the proteins was performed, respectively. (D) Data were presented as the mean±SD of three independent experiments. The level of significance was indicated by *P<0.05, **P<0.01, ***P<0.001.