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. 2019 May 28;12:4203–4211. doi: 10.2147/OTT.S196910

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© 2019 Yu et al.

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β-elemene enhanced the radiosensitivity of lung cancer and inhibited the infiltration of macrophages in tumor growth in xenograft mouse models: Female athymic nude mice aged 4–5 weeks old were used in the study. Lewis cells (5×106 in 100 μL PBS) were injected subcutaneously into the left flank of each mouse. The experiment was performed as described in Materials and Methods. The xenografts were harvested after two weeks. (A) Representative images of the xenografts. (B) Tumor volume was measured once every 2 days, and calculated as: V=(width 2×length)/2. (C) Tumor weight. (D) Immunohistochemical analysis of F4/80 and MCP-1. (E) The schematic diagram illustrating the molecular mechanisms of macrophages infiltration induced by radiation and hypoxia.