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. 2019 Apr 12;11(4):527. doi: 10.3390/cancers11040527

Figure 3.

Figure 3

PSMD3 expression is essential to maintain HER2 stability. (A) Twenty-four hours after transfection of BT474, SKBR3, and HCC-1419 with either scramble (Sc), PSMD3-Si001, or PSMD3-Si002. The cell lysates were collected and analyzed by western blot to detect the PSMD3 and HER2 protein levels. (B) Scramble, PSMD3-si001, or PSMD3-Si002 for BT474 and SKBR3 were treated with Cycloheximide (CHX) at 20 µg. The cells lysates were obtained at the indicated time points and analyzed by western blot. (C) BT474, SKBR3, and HCC-1419 were treated with Scramble, PSMD3-Si001, or PSMD3-Si002 for 24 h and the cells were lysed. The samples were resolved by SDS-PAGE and subjected to western blot analyses with indicated antibodies. (D) BT474, SKBR3 and HCC-1419 were transfected with Scramble, PSMD3-Si001or PSMD3-Si002 for 24 h, then the cells were lysed and the proteins were extracted. The samples were analyzed by western blot to detect the PSMD3, HER2, T-AKT, p-AKT, T-ERK and p-ERK, β-actin served as internal control.