Figure 4.

Silencing of PSMD3 inhibits cell proliferation and induces cell apoptosis. (A) Cell proliferation assay for BT474 and SKBR3. After transfection the cells with Scramble (Sc), PSMD3-Si001, or PSMD3-Si002, cells (2X10⁴) were seeded in a 24-well dish and cells number were counted at day 1, day 2, and day 4. Data are presented as the mean ± S.D * p < 0.05. (B) BT474 and SKBR3 were seeded in a 12-well culture plate after being treated with the indicated plasmids. After 10 days, the cells were fixed and stained with crystal violet. Data are presented as the mean ± S.D (* p < 0.05 *** p < 0.001). (C) Annexin V, cell apoptosis assay by flow cytometry. Twenty-four hours after treating the cells with the indicated plasmids, cells were collected and washed two times with PBS and stained with PE-Annexin or 7AAD. Data are presented as mean ± S.D (* p < 0.05 *** p < 0.001). (D) The expression of cell cycle and apoptotic related markers for the HER2 positive cell lines were detected by western blot (PSMD3, HER2, CDK4, CDK6, PARP, and caspase3). β-actin served as internal control.