Table 1.
Sequences of the primers used for qRT-PCR study.
| Gene symbol | Primer sequence (3′-5′) | Size (bp) | Accession no |
|---|---|---|---|
| ACTB | F: CAT CAC CAT CGG CAA CGA | 144 | XM_003124280.5 |
| R: GCG TAG AGG TCC TTC CTG ATG T | |||
| INSR | F: AGA GCG GAT CGA GTT TCT CA | 245 | XM_021083943.1 |
| R: CCA TCC CAT CAG CAA TCT CT | |||
| PPARγ | F: ACA CCG AGA TGC CGT T | 56 | XM_005669788.3 |
| R: CGA CAG GTC CAC AGA G | |||
| PPARA | F: CGA CCT GGA AAG CCC GTT AT R: GGA TCC ATC TGA TCC CGG AC |
148 | NM_001044526.1 |
| ACVR1B | F: CAT CGA GGG GAT GAT CAA GT | 211 | NM_001195322.1 |
| R: GGC AAT GTC AAT GGT GTC AG | |||
| CYP3A46 | F: ACA GCA TTT GGA GTG AAC GTC | 250 | NM_001134824.1 |
| R: CCA CTC GGT GCT TTT GTG TAT | |||
| GFPT1 | F: ATG CTC TTC AGC AGG TGG TT | 232 | XM_005662490.3 |
| R: TCT ACG GTT ACC GAT TTG GC | |||
| FFAR2 | F: TCA TGG GTT TCG GCT TCT AC | 191 | XM_021093196.1 |
| R: AAC GAT GAA CAC GAC AGT GC | |||
| TLR2 | F: ACA TGA AGA TGA TGT GGG CC | 109 | XM_005653577.3 |
| R: TAG GAG TCC TGC TCA CTG TA | |||
| TLR3 | F: TAG AGA CAT GGA TTG CTC CC | 435 | NM_001097444.1 |
| R: AAC TTC TGG AAT GCA GGT CC | |||
| TLR4 | F: CTC TGC CTT CAC TAC AGA GA | 322 | NM_001293316.1 |
| R: CTG AGT CGT CTC CAG AAG AT | |||
| EPCAM | F: GCG ATA GCG ATT GTT GCT GG | 106 | NM_214419.1 |
| R: CCC TAT GCA TCT CGC CCA TC | |||
| SELL | F: GTG ATG CAG GGT ACT ACG GG | 108 | NM_001112678.1 |
| R: AGA ACT TGC CCA AAG GGT GA | |||
| SAA2 | F: AGA GCC TAC TCG GAC ATG AGA GA | 65 | NM_001044552.1 |
| R: CCC CGG GCA TGG AAG TAC | |||
| CCL5 | F: CCA GCA GCA AGT GCT CCA T | 60 | NM_001129946.1 |
| R: ACA CCT GGC GGT TCT TTC TG | |||
| CXCL2 | F: CCG GGA CCC CAC TGT GA | 62 | NM_001001861.2 |
| R: CAAACTTCCTGACCATTCTTGAGA | |||
| CFB | F: CCT CGG GCT CCA TGA ATA TC | 56 | NM_001101824.1 |
| R: TGC CCC AAT GCT GTC TGA T | |||
| C3 | F: CCA ACA GGG AGT GCA ACG A | 70 | NM_214009.1 |
| R: TGA CTC CGT GTC TGG GAC TTG | |||
| CSF1 | F: CCA ACA GGG AGT GCA ACG A | 147 | NM_001244523.1 |
| R: TGA CTC CGT GTC TGG GAC TTG | |||
| TGFB3 | F: TTG CTA AAT GCT CCA GCC AG | 90 | NM_214198.1 |
| R: GCC TCC GCC TGT AGA ACA AG | |||
| TNFAIP3 | F: CCC TGG GGC ATT ATG GGT TT | 60 | NM_001267890.1 |
| R: CCT CAC ACG TTG TAG CAC CT | |||
| CCL2 | F: CCT CCC TGG AAA GCC AGA A | 58 | NM_214214.1 |
| R: GTG CCA CAA GCT TCC TCA CTT | |||
| TNFα | F: CGA CTC AGT GCC GAG ATC AA | 58 | JF831365.1 |
| R: CCT GCC CAG ATT CAA AG | |||
| IL8 | F: GCT CTC TGT GAG GCT GCA GTT | 62 | NM_213867.1 |
| R: TTT ATG CAC TGG CAT CGA AGT T | |||
| IL6 | F: TGG ATA AGC TGC AGT CAC AG | 109 | NM_214399.1 |
| R: ATT ATC CGA ATG GCC CTC AG | |||
| IL1α | F: AGA ATC TCA GAA ACC CGA CTG TTT | 62 | NM_214029.1 |
| R: TTC AGC AAC ACG GGT TCG T | |||
| IL1β | F: GCC CTG TAC CCC AAC TGG TA | 61 | NM_001302388.2 |
| R: CCA GGA AGA CGG GCT TTT G |
F, Forward; R, Reverse; bp, base pair.