Figure 6.

ctDNA dynamics in patients without tumor growth during NAC. Serial ctDNA quantification using patient-specific mutation panels. Limit of detection calculated as mean across all time points (see Methods). Indeterminate time points have either 0 or a statistically insignificant number of mutant reads; those points are plotted at limit of detection. Indeterminate points are labeled by mutant reads/total reads. Error bars ± SEM. (A) Six-mutation panel for BCR492. (*) Paclitaxel + trastuzumab + HSP90 inhibitor, which was discontinued because of toxicity issues. (B) Twenty-five-mutation panel for BCR494. (Ganit) Ganitumab, a monoclonal antibody targeting IGF-1R pCR, (gy) gray unit of radiation dose. (C) Fifteen-mutation panel for BCR495 pCR. (D) Eighteen-mutation panel for BCR503. (Pertuz) Pertuzumab.