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. 2019 Apr 3;160(7):1561–1572. doi: 10.1210/en.2019-00212

Figure 2.

Figure 2.

In vivo regulation of Sfrp mRNA levels in GCs by gonadotropins. Immature (21- to 26-d-old) WT female mice were either injected (or not) with eCG (5 IU, IP) and GCs isolated at the indicated times, or else they were injected with eCG 44 to 48 h prior to the administration of hCG (5 IU, IP) and GCs isolated at the indicated times. mRNA levels for each Sfrp gene were determined by RT-qPCR and normalized to the housekeeping gene Rpl19 (n = 3 to 4 animals per time point). Data are expressed as means ± SEM. Different letters show statistically significant differences between groups (P < 0.05).