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. 2019 Apr 3;160(7):1561–1572. doi: 10.1210/en.2019-00212

Figure 4.

Figure 4.

Loss of Sfrp4 does not affect cumulus expansion. (A) Expression pattern of genes known to be regulated during COC expansion. Immature mice of the indicated genotypes were treated with eCG for 48 h, followed (or not) by hCG for up to 20 h. mRNA levels were determined in isolated COCs by RT-qPCR and normalized to the housekeeping gene Rpl19. For all analyses, n = 5 samples per genotype per time point, with each sample consisting of a pool of COCs collected from two to five animals. All data are expressed as means ± SEM. *P < 0.05, significant difference vs control at the same time point. (B) Representative photomicrographs and diameter measurements of COCs collected by flushing the oviducts of mice of the indicated genotypes 20 h following an ovulatory dose of hCG. n = 10 to 11 COCs per genotype. Scale bar, 100 μm. Data are expressed as means ± SEM.