Fig. 11. Comparison of the repeated fluorescence images of HeLa cells stained with (a) 5M and (b) LysoTracker™ Red under irradiation with a confocal laser at 561 nm (WLL, output power 70%, AOTF 2%). Each number indicates the number of recorded confocal images. The rectangle (bottom, left in each picture) is the cutout of the image at n = 1. Scale bar: 2 μm. (c) Plots of integrated fluorescence intensities (I) relative to the initial value (I0) as a function of the number of recorded images.
