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. 2019 May 31;11:5133–5146. doi: 10.2147/CMAR.S203656

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© 2019 Yang et al.

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OR HCC cells exhibit low expression of Cx32. (A) Western blot was performed to assess protein expression levels of Cx26, Cx32, and Cx43 in parental HepG2, Huh7, SMMC-7721 cells, and OR counterpart cells. (B) Quantitative results for Cx32 protein expression. Bar graphs were derived from densitometric scanning of blots for Cx32. (C) Decreased mRNA level of Cx32 was detected in OR HCC cells compared with parental HCC cells, as shown by qRT-PCR. (D) Immunofluorescent staining revealed decreased expression of Cx32, by showing that weaker fluorescence intensities for positive signals were observed, in SMMC-7721/OXA cells compared with parental SMMC-7721 cells (original magnification, ×200). OR HCC cells were cultured in cell growth medium containing 5 μg/mL, 4 μg/mL, and 8 μg/mL OXA for HepG2, Huh7, and SMMC-7721, respectively. **P<0.01 vs parental control.

Abbreviations: Cx, connexin; HCC, hepatocellular carcinoma; OR, OXA-resistant; OXA, oxaliplatin.