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. Author manuscript; available in PMC: 2019 Jun 5.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4407–4420. doi: 10.1167/iovs.07-0432

Figure 2.

Figure 2.

Effects of Rz432 on MnSOD expression in RPE-J cells. (A) Quantification of MnSOD transcript levels measured in triplicate with reverse transcription PCR. β-Actin amplified in the same reactions was used as an internal control. PCR products were quantitated by SYBR green staining. A significant decrease in MnSOD transcripts was observed by 24 hours after treatment with Rz432 (P < 0.05). (B) Western blot for MnSOD protein levels from Rz432 or GFP-treated cells. MnSOD mRNA and protein levels returned to normal after 4 days, because of the transient transfection. This experiment was performed twice, with essentially identical results.