Table 1.
Nitrated Proteins in SODRZ-Treated Mouse Posterior Eye Cup Identified by LC-MS/MS
| Gel Band No. | Protein Name | Matched Peptide | Molecular Weight | Swiss-Prot Accession No. | Nitrotyrosine-Containing Peptide | Ion Score | E-Value Swiss-Prot (229K) | E-Value NCBI (4396K) |
|---|---|---|---|---|---|---|---|---|
| 5 | Transducin-like enhancer protein 3 (mouse) | 1 | 83,160 | Q08122 | K.30DEFQFLQAQ@Y*HSLK43.V | 19 | 6e-08 | 4e-07 |
| 16 | Elastase-1 precursor (rat) | 1 | 28,822 | P00773 | K.52WY*HTC#GGTLIR62.Q | 15 | 7e-06 | 5e-05 |
| 16 | Fibroblast growth factor receptor 2 precursor (mouse) | 1 | 91,984 | P21803 | R.650DIN@NIDY*YKK659.T | 18 | 2e-04 | 0.002 |
| 5 | Early development regulatory protein 2 (p36) (mouse) | 1 | 89,799 | Q9QWH1 | K.592KY*AQ@GFLPEK601.P | 33 | 0.001 | 0.009 |
| 12 | Zinc finger protein 277 (human) | 1 | 51,582 | Q9NRM2 | R.87Y*ILY*WRKR94.F | 22 | 0.006 | 0.052 |
| 4 | LIM/homeobox protein Lhx8 (mouse) | 1 | 40,670 | O35652 | R.152RY*GTRCSR159.C | 20 | 0.05 | 0.34 |
| 18 | Growth factor independence 1 (mouse) | 1 | 45,845 | P70338 | R.249LLLGGGSY*K257.C | 16 | 0.076 | 0.52 |
| 18, 20 | Apoptosis-inducing factor 2 (mouse) | 1 | 40,609 | Q8BUE4 | K.247IN@SSAY*R253.S | 17 | 1.1 | 7.6 |
The sequence of each peptide was analyzed by BLAST against the Swiss-Prot and NCBI database and corresponding species. The sequence of each nitrated peptide matched exactly to the sequence of each protein, and that protein was the only one to be matched in Swiss-Prot database and corresponding species. The same protein was also exactly matched in NCBI database and corresponding species. E-Value, expectation value, the number of alignments with scores ≥ than the associated score expected to occur in a database search by chance. Y*, nitrated tyrosine; C#, carbamidomethylated Cys; N@, deamidated Asn; Q@, deamidated Gln.