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. 2019 May 29;10:1177. doi: 10.3389/fmicb.2019.01177

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Copyright © 2019 Su, Jin, Wu, Fei, Li, Guo, Feng and Xiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cross-reactivity of polyclonal antibodies (PcAb) among MEDLE proteins. (A) Western blot analysis of cross-reactivity among MEDLE proteins, using 1 μg of CpMEDLE-5 (lane 1), CpMEDLE-3 (lane 2), ChMEDLE-1 (lane 3), CpMEDLE-1 (lane 4) and CpMEDLE-2 (lane 5) and PcAb to CpMEDLE-3 (left panel), CpMEDLE-5 (middle panel) and ChMEDLE-1 (right panel). (B) ELISA analysis of polyclonal antibodies to each MEDLE protein, with data being normalized using the relative absorbance of each antibody to the corresponding protein.