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. 2019 May 29;10:1177. doi: 10.3389/fmicb.2019.01177

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Copyright © 2019 Su, Jin, Wu, Fei, Li, Guo, Feng and Xiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of CpMEDLE-3 in sporozoites and developmental stages of Cryptosporidium parvum. (A) Western blots analysis of native protein from C. parvum sporozoites for CpMEDLE-3, using polyclonal antibodies (left panel), post-immune sera (middle panel) and pre-immune sera (right panel). Lane M: molecular weight markers; lane 1: crude protein extracted from sporozoites; lane 2: purified CpMEDLE-3 protein. (B) Expression of CpMEDLE-3 on C. parvum sporozoites (top panel) and intracellular developmental stages in HCT-8 cell cultures at 24 h (middle panel) and 48 h (bottom panel). The images were taken under differential interference contrast (DIC), with nucleus counter-stained with 4′,6-diamidino-2-phenylindole (DAPI), parasites stained by immunofluorescence with Alexa 594-labled CpMEDLE-3 (CpMEDLE-3), and superimposition of the three images (Merged). Bars = 5 μm.