Fig. 2.

Intraperitoneal injection of hUCB-MSCs reduces the intestinal inflammation and increases the secretion of WRS. Mice were administered with 3% DSS solution for seven days and infused intraperitoneally hUCB-MScs at day one. The mice were sacrificed at day 14 for more ex vivo examination. (A) Left: H&E-stained images of colon, bar = 500 μm (upper), 200 μm (lower). Right: Histopathological severity was quantified based on the criteria. (B) Left: PSR-stained images of colon, bar = 500 μm (upper), 200 μm (lower). Right: Fibrotic areas were calculated. (C) Myeloperoxidase (MPO) activity was detected in the colon of mice. (D) The level of secreted WRS in the serum of mice was determined by Western blot analysis and quantified. n = 3–9 mice per group. *P < 0.05, ***P < 0.001. Results are shown as the mean ± SEM.