Fig. 4.

IFN-γ plays a crucial role in WRS secretion of hUCB-MSCs for protection against excessive inflammation. (A) The mice were sacrificed at day 14 and serum samples were collected for more ex vivo examination. Level of IL-6, IL-12, TNF and IFN-γ in the serum of mice was measured by CBA analysis. (B–D) hUCB-MSCs were treated with indicated cytokines including IFN-γ for 24 hours. Expression level of WRS was determined in (B) the cell lysates and (C) cell-cultured media. (D) Inhibitory effect of IFN-γ-primed hUCB-MSCs on proliferation of hUCB-MNCs was detected by BrdU ELISA assay. n = 4–6 mice per group. *P < 0.05, **P < 0.01, ***P < 0.001. Results are shown as the mean ± SEM.