Fig. 2.

(A) Penetration time-course of purified dsRED-9K was checked. HEK293 cells were plated in a 12-well plate and treated with 20 μg of dsRED-9K in their culture medium for 24 hrs. Fluorescent images were acquired 8 times in 24 hrs with matching exposure. (B) Images were analyzed by CellProfiler (version 2.1.1; cellprofiler.org) for measuring the fluorescence intensity. Three independent fields from each time point were used with the matching exposure. Each cell was identified by object identification modules, followed by measurement of red fluorescent intensity.