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. 2019 May 29;10:1200. doi: 10.3389/fmicb.2019.01200

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Copyright © 2019 Born and Pfeifer.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effect of the 5′-UTR on the expression during growth. The effect of the 5′-UTR_A, 5′-UTR_D, and 5′-UTR_F on the expression of the corresponding mRNA was quantified throughout growth. The fluorescence of the transformants measured at OD₆₀₀ 0.3, 0.6, 1.2, and 3.0 (42°C) is given in arbitrary units (AU). The autofluorescence of Hfx. volcanii was subtracted from each value. Each experiment was performed in triplicates of three different biological samples.