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. 2019 May 15;132(10):jcs226886. doi: 10.1242/jcs.226886

Fig. 5.

Fig. 5.

Modulation of CFTR transcytosis. (A,B) CFTR recycling, apical density and transcytosis were measured in CFBE cells silenced for the indicated protein. Results are expressed as a percentage of that seen with the control siRNA (CTL). (C) STX3 may contribute to the basolateral missorting of newly synthesized CFTR. CFTR apical transcytosis was measured after 24 h dox-OFF (i.e. after washout) in CFBE cells transfected with control (CTL) or STX3 siRNAs (siSTX3). (D) CFTR apical transcytosis is MT dependent. CFTR and TfR transcytosis was determined in CFBE cells transfected with control (CTL) or Myo5B-specific siRNAs (siMyo5B 1 or 2) or pretreated for 30 min and during transcytosis with 33 µM nocodazole (Noco) or DMSO. Data are means±s.e.m. on each panel, parentheses indicate the number of independent experiments. *P<0.05; **P<0.01; n.s., not significant.