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. 2019 May 17;12(5):dmm038109. doi: 10.1242/dmm.038109

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© 2019. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 4. — The effect of TDP-43^A315T expression on synaptic receptor expression. (A-H) YFP:WT and YFP:TDP-43^A315T primary cortical neurons at 10 DIV were labeled for postsynaptic receptors and GFP (antibody against GFP enhances endogenous YFP fluorescence). Immunocytochemistry labeling for the glutamate receptor subunits (red, first column) GluR1 (A), GluR2 (C) and GluR4 (E) and the postsynaptic protein PSD-95 antibody (G), and GFP antibody (green, second column); colocalization (black, third column) was identified in ImageJ and overlaid onto GFP fluorescence images (white; fourth column). (B,D,F) The ratio of GluR1 to GFP was significantly increased (B), whereas there was no significant differences in the ratio of GluR2 to GFP (D) and GluR4 to GFP (F) in the TDP-43^A315T neurons in comparison to WT controls. (H) There was no significant difference in the ratio of PSD-95 to GFP in the TDP-43^A315T neurons in comparison to WT controls. n=3 biological replicates. *P<0.05 (Student's t-test). Data are mean±s.e.m. Scale bar: 10 μm.